Bacterial cell wall degradation by a staphylococcal autolysin
نویسندگان
چکیده
منابع مشابه
Structural Basis of Cell Wall Cleavage by a Staphylococcal Autolysin
The major autolysins (Atl) of Staphylococcus epidermidis and S. aureus play an important role in cell separation, and their mutants are also attenuated in virulence. Therefore, autolysins represent a promising target for the development of new types of antibiotics. Here, we report the high-resolution structure of the catalytically active amidase domain AmiE (amidase S. epidermidis) from the maj...
متن کاملActivity of the major staphylococcal autolysin Atl.
The major autolysin of Staphylococcus aureus (AtlA) and of Staphylococcus epidermidis (AtlE) are well-studied enzymes. Here we created an atlA deletion mutant in S. aureus that formed large cell clusters and was biofilm-negative. In electron micrographs, the mutant cells were distinguished by rough outer cell surface. The mutant could be complemented using the atlE gene from S. epidermidis. To ...
متن کاملOptical configuration of staphylococcal cell wall serine.
Studies on the amino acid content of the cell wall of gram-positive bacteria have shown them to be composed of relatively few amino acids. One of the principal amino acids found in the cell wall of certain staphylococci is serine (1, 2, 5, 6). Although the optical configuration of staphylococcal cell wall serine has not been determined, Whitney and Grula (8) found that Micrococcus lysodeikticus...
متن کاملLytU-SH3b fusion protein as a novel and efficient enzybiotic against methicillin-resistant Staphylococcus aureus
Methicillin-resistant Staphylococcus aureus (MRSA) is a challenging infectious agent worldwide. The ever growing antibiotic resistance has made the researchers to look for new anti-staphylococcal agents. Autolysins are staphylococcal enzymes that lyse bacterial cell wall for cell division. Autolysins can be used as novel enzybiotics (enzymes have antibiotic effects) for staphylococcal ...
متن کاملBacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR.
The ability to characterize bacterial cell-wall composition and structure is crucial to understanding the function of the bacterial cell wall, determining drug modes of action and developing new-generation therapeutics. Solid-state NMR has emerged as a powerful tool to quantify chemical composition and to map cell-wall architecture in bacteria and plants, even in the context of unperturbed inta...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2011
ISSN: 0108-7673
DOI: 10.1107/s0108767311097819